摘要
目的：为了制备高效的，特异的抗人喉癌／抗蓖麻毒素A双功能单克隆抗体，观察其对Hep－2细胞株杀伤作用。方法：选用BALB／C鼠，分别行蓖麻毒素A和人喉鳞状细胞癌腹腔免疫后，提取抗体，经与骨髓瘤细胞融合，制备成抗人喉癌／抗蓖麻毒素A双特异性单克隆抗体。结果：该双功能抗体与喉癌组织的特异性结合率为87％，对Hep－2细胞半数抑制浓度比游离环磷酰胺强40倍。结论：采用二次杂交瘤技术制备的双功能抗体优于化学偶联法，抗体效价具有均匀性和理想的信噪比，抗体易于标准化。
To investigate the preparation of a human laryngeal cancerbispecific antibody. Methods: The second set hybrid tumor technology was applied to prepare an anti-human laryngeal cancer and anti-castor toxin A bispecific monoclonal antibody. Results: The specific conjugation rate between bispecific monoclonal antibody and laryngeal cancer tissue was 87%. The half inhibition concentration to Hep-2 cell was 40 times stronger than that of free cyclophosphamide. Conclusion:The second set hybrid tumor technology to prepare the bispecific antibody had a homogeneous titer,an idealsignal-to-noise ratio and was easy to be standardized.
出处
《耳鼻咽喉（头颈外科）》
2000年第3期164-166,共3页
Chinese Arch Otolaryngology-Head Neck Surg
基金
国家自然科学基金!39470747
关键词
喉肿瘤
抗体
制备
肿瘤抗体
Laryngeal neoplasms
Carcinoma
Antibodies，noplasm
