喉癌和癌旁组织的端粒酶活性检测

Telomerase activity in laryngeal carcinoma and the tumor-adjacent tissues

目的：探索端粒酶活性与喉癌发生发展的关系。方法：采用重复序列扩增法（TRAP－PCR）检测56例手术切除的喉癌组织和癌旁粘膜组织的端粒酶活性。喉癌组织均经病理证实，喉癌旁粘膜组织中有正常喉粘膜41例，轻度不典型增生15例。结果：喉癌组织端粒酶活性阳性率为91．07％（51／56），正常喉粘膜和轻度不典型增生喉粘膜的阳性率分别为9．76％（4／41）和33．33％（5／15），喉癌组织和癌旁粘膜组织中端粒酶活性阳性率有显著差异（P<0．01）。癌旁上皮端粒酶活性阳性的9例患者其喉癌组织端粒酶活性皆为阳性。结论：端粒酶激活与喉癌的发生发展有密切关系，并可作为喉癌分子诊断的肿瘤标记物。

Objective:To investigate the possible relationship between telomerase expression and progression of laryngeal carcinoma. Methods:Fifty-six laryngeal carcinoma and 56 corresponding tumor adjacent epithelium specimens were measured by means of a telomeric repeat amplification protocol (TRAP) assay for telomerase activity. All the cancer tissues were pathologically confirmed. Forty-one cases of normal laryngeal epithelium and 15 mild dysplasia constituted the tumor adjacent specimens. Results: The positive expression rate of telomerase in laryngeal cancer,mile dysplasia and normal epithelium tissues was 91.07%, 33. 33% and 9. 76%,respectively. A difference of statistical significance was found between the expression rate of the cancer and that of the near by epithelium (P<0.01). Positive expression was also found in the cancer tissues of these 9 cases,which showed positive results in the tumor adjacent tissues. Conclusions:Telomerase expression likely contributed to the progression of laryngeal carcinoma and might be a tumor marker of laryngeal carcinoma. Further study is needed to evaluate prognostic value of telomerase activity in laryngeal carcinoma.