实验性肝硬化血浆内毒素、一氧化氮与内皮素的含量变化及其意义

The Changes of Plasma Endotoxin, Nitric Oxide and Endothelin in Experimental Cirrhotic Rats and Their Significance

目的 :通过观察实验性肝硬化形成过程中内毒素、一氧化氮 (NO )与内皮素 (ET )的动态变化 ,探讨其与肝硬化高动力循环状态的关系。方法 :采用鲎试剂基质显色法、镉还原比色法和放射免疫分析法测定了四氯化碳 (CCI4 )诱发大鼠肝硬变形成过程中血浆内毒素、NO、ET含量变化。结果 :在肝硬化进程中血浆内毒素、NO含量呈上升趋势且明显高于正常对照组 (P <0 .0 1) ,血浆ET含量呈下降趋势且明显低于正常对照组 (P <0 .0 1) ,均与肝功受损相关。结论 :血浆内毒素、NO、ET水平反映肝硬化的严重程度 。

Purpose: In order to assess the role of vasoactive substances in hemodynamic disturbances, we observed the dynamic changes of plasma endotoxin, nitricoxide (NO) and endothelin (ET) in rats during the development of experimental cirrhosis. Methods: The plasma concentration of endotoxin, NO and ET were measured by the means of limulus amoebocyte lystate assay, griess assay and radioimmnoassay in rats at 3、 6、 9、 12 week after carbon tetrachloride(CCl 4)administration. Results: The plasma levels of endotoxin and NO in four groups of cirrhotic rats were significantly higher than normal controls (P<0.01). The plasma level ET of decreased markedly compared with those of normal controls (P<0.01). Both of them were proportional to the various degrees of liver damage. Conclusion: The plasma level of endotoxin, NO and ET can reflect the severity of cirrhosis,and hyperdynamic circulation may occur with the development of experimental cirrhosis.