GST-π在胃癌及癌旁正常组织表达及预后关系探讨

Study on glutathione s transferase-π(GST-π)expression in stomach carcinoma and its adjacent tissue and the relationship with prognosis

目的 探讨谷胱甘肽转移酶π(Glutathione s Transferase π .GST π)与胃癌的分型、分期及预后与多药耐药的关系。方法 应用鼠抗人GST π单克隆抗体对 80例胃癌进行免疫组化研究。结果 GST π表达与临床分期无关 (P>0 .0 5 ) ,与胃癌组织分化程度无关 (P >0 .0 5 )。不同生存年段中GST π的表达无统计学差异 (P >0 .0 5 )。但GST π在癌旁正常组织中的阳性表达与生存期有统计学意义 (P <0 .0 5 )。同时还发现GST π在瘤组织中的表达强度与平均生存期有关 (P <0 .0 5 )。结论 GST π表达与胃癌的分期无关 ,与分型无关 ,不宜作为恶性度的指标 ,但其在瘤组织中的表达强度及在癌旁正常组织中的表达均与生存期有关 。

? Objective To investigate the patterns of GST π expression and its influences on prognosis.Methods 80 cases of paraffin embedded stomach cancer tissues were stained immunohistochemically for GST π using monoclonal antibody after heated under high pressure.Results GST π experessed in 91.1% cancer tissues and 87.5% in normal tissue.The expression was associated with tumor clinical stage (P<0.05) and defferentiation.But its experession in normal tissue was postively correlated with survial time, the level of expression in tumor tissue also correlated significantly with prognosis.Conclusions Expression of GST π is related to the biologic behaviors of stomach cancer.Immunohistochemical assessment of GST π expression in stomach carcinoma and in normal itssue might be useful in choosing suitable chemotherapy and predicting prognosis. 〔