胃癌病变中血小板源生长因子的表达

Experession of platelet derived growth factor in gastric carcinoma and its significance

目的 探讨血小板源生长因子 (PDGF)在胃癌发生发展中的作用。方法 对 18例胃癌、3 6例慢性胃炎和15例对照组 ,应用快速尿素酶试验和Warthin starry法检测Hp ;采用细胞生物活性法测定胃癌、胃炎组织和胃癌细胞株MGC80 3培养上清液中的PDGF活性。结果 Hp感染率在胃癌组和胃炎组无显著性差异。胃癌、胃炎组织和胃癌细胞培养上清液中均能测到PDGF活性 ,胃癌组要明显高于胃炎组。PDGF可明显促进胃癌细胞的体外增殖 ,且呈剂量依赖关系 ,抗 PDGF抗体却可明显抑制胃癌细胞的增殖。

? Objective To investigate the role of platelet derived growth factor (PDGF)in the pathogenesis of gastric carcinoma.Methods Hp was examined in 18 patients with gastric carcinoma,36 patients with chronic gastritis and 15 controls by rapid urease test and Warthin Starry method.Bioactivity assay was used to measure the activity of PDGE in the tissue of gastric carcinoma and the supernate of gastritis and gastric carcinoma cell line MGC803.Results There was no significant difference in Hp infection rate between gastric carcinoma and gastritis.PDGF activity was expressed in gastric carcinoma,gastritis and MGC803 cell line,and its expression level was higher in gastric carcinoma than in gastritis.PDGF could obviously promote MGC83 cell prolifeation and showing dose dependent manner;but anti PDGF antibody obviously could suppress MGC803 cell proliferation.Conclusions PDGF may be associated with genesis of gastric carcinoma. 〔