摘要
目的明确聚集性食源性腹泻暴发的病原。方法根据现场流行病学调查,对患者肛拭子标本18份,粪便标本2份,厨师粪便标本1份,进行分离培养,对分离到的可疑病原菌进行生化鉴定,采用Real-time PCR技术检测可疑病原菌的ipaH毒力基因,同时进行脉冲场凝胶电泳(PFGE)分型分析。结果分离到11株血清型为O136∶K78的肠侵袭性大肠埃希菌,除1份肛拭子外,其余7份患者肛拭子、3份粪便标本Real-time PCR均阳性;除1株菌外,其余10株菌株的PFGE带型一致。结论具有侵袭性相关基因ipaH的O136∶K78肠侵袭大肠埃希菌是本次聚集性腹泻暴发的主要原因。
Objective To determine the pathogen of a foodborne outbreak of diarrhea.Methods On the basis of epidemiological investigation,eighteen rectal swabs and 2 stool samples from the patients and 1 stool sample from a cook were collected.After pathogen isolation and culture,the biochemical identification of the isolated strains was performed,the invasive plasmid antigen H gene（ipaH） of the positive strains were detected by real-time PCR and the pulsed-field gel electrophoresis homology analysis was conducted.Results Eleven strains of enteroinvasive Escherichia coli O136∶K78 were isolated.All the samples,except 1 rectal swab,were positive in real time PCR,and the PFGE patterns of all the strains,except one,were consistent.Conclusion The enteroinvasive Escherichia coli O136∶K78 with ipaH gene was the pathogen to cause the outbreak of diarrhea.
出处
《疾病监测》
CAS
2012年第9期726-728,共3页
Disease Surveillance
