2012年辽宁省铁岭市食源性致病菌监测中检测出1例诺如病毒

Detection of norovirus in foodborne pathogen surveillance in Tieling,Liaoning,2012

目的了解辽宁省铁岭市水产品中食源性致病菌的污染状况,有效预防控制食源性疾病的发生。方法应用反转录-聚合酶链反应(RT-PCR)对2012年第一季度食源性致病菌常规监测采集到的6份水产品进行检测,对阳性PCR产物进行基因测序。结果 6份水产品中,有1份检测出诺如病毒RNA阳性。测序结果分析显示,该阳性标本的诺如病毒VP1全基因序列测定和分析显示为GⅡ-4型诺如病毒。结论铁岭市首次在食源性致病菌日常监测中检测出诺如病毒GⅡ-4型。因此应加大对生食水产品的监督检查力度,避免因诺如病毒引起的病毒性胃肠炎暴发。

Objective To understand the contamination status of foodborne pathogens in aquatic products in Tieling for the effective prevention and control of foodborne diseases.Methods Reverse transcription-polymerase chain reaction（RT-PCR）.was conducted to detect 6 aquatic product samples collected during the routine surveillance in Tieling in the first quarter,2012,and gene sequencing was done for the positive PCR products.Results Norovirus was detected in 1 of the 6 aquatic product samples and the sequence analysis indicated that it belonged to GⅡ-4 genotype.ConclusionIt was the first time to detect norovirus GⅡ-4 genotype in the routine surveillance of foodborne pathogens in Tieling.It is necessary to strengthen the inspection and detection of aquatic products to prevent the outbreak of norovirus related viral gastroenteritis.