低浓度乙醇预防胆管癌术后复发的可行性研究

Feasibility study of low concentration ethanol in preventing postoperative recurrence of cholangiocarcinoma

目的探讨低浓度乙醇预防胆管癌术后复发的可行性。方法以不同浓度乙醇直接作用于培养的胆管癌细胞系QBC939细胞,MTT法筛选抑制肿瘤细胞增殖的乙醇浓度。经不同浓度乙醇预处理15min的QBC939细胞,接种于裸鼠皮下,通过裸鼠种植瘤模型筛选乙醇抑制肿瘤细胞体内生长的安全浓度。将筛选出的安全浓度乙醇蒸馏水溶液注射于大鼠腹腔,观察其对动物生命体征及腹腔脏器的影响。结果乙醇对胆管癌细胞系QBC939细胞的半数抑制浓度(IC50)为1.8%-2.2%,0.5%以上浓度的乙醇即可显著抑制肿瘤细胞生长;15%乙醇预处理过的肿瘤细胞接种于裸鼠皮下,15d后未见肿瘤结节生长;15%浓度乙醇蒸馏水溶液10ml注射大鼠腹腔后未见其对动物生命体征及腹腔脏器造成显著影响。结论低浓度乙醇可有效抑制胆管癌细胞生长,对动物生命体征及腹腔脏器安全,具有术中浸泡腹腔预防胆管癌术后复发的潜在应用价值。

Objective To study the feasibility of low concentration ethanol in preventing the relapse of cholangiocarcinoma after operation.Methods Human cholangiocarcinoma QBC939 cells were cultured with ethanol at different concentrations for 15 minutes.The concentration of ethanol inhibiting the proliferation of QBC939 cells was measured by MTT assay.After pretreated with ethanol at different concentrations for 15 minutes,the QBC939 cells were inoculated into the subcutaneous tissue of nude mice.A nude mouse tumor model was established to determine the security ethanol concentration inhibiting the proliferation of QBC939 cells.Effect of security distilled ethanol solution injected into abdominal cavity of rats on their vital signs and abdominal organs was observed.Results The IC50 of ethanol on proliferation of QBC939 cells was 1.8%-2.2%.Ethanol with its concentration over 5% could significantly inhibit the proliferation of QBC939 cells.No tumor nodule was found 15 days after 15% ethanol–pretreated QBC939 cells were inoculated into subcutaneous layer of rats.10ml 15% ethanol distilled solution injected into abdominal cavity had no significant effect on vital signs and abdominal organs of rats.Conclusion Low concentration ethanol can effetely inhibit the proliferation of QBC939 cells without affecting the vital signs and abdominal organs of rats,and is thus of a potential application value in preventing recurrence of cholangiocarcinoma after operation.