氧氟沙星荧光敏感膜的研究

An Ofloxacin Sensing Membrane Based on Fluorescence Quenching of Fluorescent Reagent

研制了对氧氟沙星有可逆响应的荧光敏感膜，将合成的亲酯性荧光试剂（环己基氨基，环己基亚氨基）芘丁酸甲酯，固定于醋酸纤维素中作为分析物识别器，测定氧氟沙星浓度．方法的回收率为96．0％～106．7％．日内、日间RSD为1．0％～2．1％，在2．0～50．0μg／ml浓度范围内至线性，相关系数0.9995，最低检出限为0．046μg／ml．

A new ofloxacin sernsing membrane based on fluorescence quenching is reported.The sensitive optrode was combined with a membrane onto which was immobilized fluoroprobe- (cyclohexylamino, cyclohexylimide)-methyl 1-pyrenebutyrate. It can montor the concentration of ofloxacin. The recovery of the method was 96. 0 % ~ 106. 7 %. The relative standard deviations for within-day and between-day were 1. 0%~ 2. 1 %. The calibration curve was lincar in the range from 2.0μg/ml with γ=0.999 5. The detection limit of this method was 0. 046 μg/ml.