宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织Shh及其受体Ptch1 mRNA表达的影响

Intra-amniotic androgen exposure on expressions of Shh and Ptch1 mRNA in embryo and pre-term rat genital tissue

目的:观察宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织Shh及其受体Ptch1 mRNA表达的影响。方法:SD妊娠大鼠随机分为氟他胺组(Flu组)和生理盐水组(对照组),分别于孕12～16d腹部皮下注射Flu或生理盐水100mg/(kg·d),2组均于胚胎19d及出生后1、4、7d各随机取8只大鼠,应用RT-PCR技术检测各组胎鼠及新生大鼠阴茎组织Shh基因及其受体Ptch1的表达。结果:2组动物Shh mRNA的表达在孕19d～出生后4d均随鼠龄增加表达逐渐增强,但Flu组在孕19d及出生后1、4、7d均低于对照组(P<0.05)。对照组Ptch1 mRNA的表达在孕19d～出生后7d随鼠龄增加无变化(F=3.231,P=0.143),而Flu组有增强的趋势(F=8.922,P=0.030),且Flu组在孕19d及出生后1、7d与对照组相比差异有统计学意义(P<0.05)。结论:宫内抗雄性物质暴露可能通过调控Shh基因及其受体Ptch1的表达引起阴茎组织发生发展中尿道襞间充质细胞与上皮细胞转化异常,尿道板腔化受阻,进而导致尿道下裂的发生。

Aim：To investigate the expressions of sonic hedgehog（Shh） and Ptch1 mRNA in genital tissue of embryo and pre-term rats exposed to intra-amniotic androgen.Methods：Pregnant SD rats were randomly divided into two groups：normal saline group and flutamide（Flu） group.Normal saline or Flu were injected into the rats from gestational days 12 to 16,respectively.The genital tissue from the 2 groups on gestational day 19 and postnatal day 1,4,and 7 was removed. Shh and Ptch1 mRNA levels were measured by semi-quantitative reverse transcription PCR.Results：The level of Shh mRNA increased in both two groups from gestational day 19 to postnatal day 4. However, in Flu group,it had significant difference from controls on gestational day 19 and postnatal days 1,4 and 7（P0.05）.The level of Ptch1 mRNA in Flu group increased from gestational day 19 to postnatal day 7（F=8.922,P=0.030）,which had significant differences from controls on gestational day 19,and postnatal day 1 and 7（P0.05）.Conclusion：The results suggest Flu exposure regulate the expressions of Shh and Ptch1 mRNA,and perhaps contribute to the pathogenesis of hypospadias.