针刺内关治疗缺血性中风微循环最佳参数筛选研究

Study on Screening Optimal Parameters of Microcirculation of Ischemic Stroke Treated by Needling Neiguan

目的:了解针刺参数对针刺微循环疗效的影响。探讨:"醒脑开窍"针刺法中内关穴最佳刺激参数。方法:复制大鼠大脑中动脉缺血模型(MCAO),以针刺频率的慢、中、快(1、2、3次/秒)和针刺时间的短、中、长(5s、60s、180s)的两因素三水平针刺参数搭配组合9种针刺参数组以捻转手法刺激"内关穴",以微循环(输入枝管径、输出枝管径、输入/输出)为效应指标,通过多因素方差分析明确时间、频率以及二者交互作用的显著性,通过正交设计分析不同针刺参数组作用于"内关穴"对MCAO大鼠微循环的影响。结果:频率对微循环有显著影响,时间和交互作用的影响较弱。输入枝管径的最优组合是快频率,中时间组(3次/秒,60秒);输出枝管径的最优组合是快频率,长时间组(3次/秒,180秒);输入/输出的最优组合为快频率,短时间组(3次/秒,5秒),可见临床上针刺在改善微循环,增加微循环灌注量上必须保证快频率,才能取得较满意的疗效。

Objective ： To know the acupuncture parameters on the effect of microcirculation, this paper discusses the best stim- ulation parameters. Methods ： The brain ischemia model in rats （MCAO） was copied. The frequency of acupuncture （ 1,2,3 times per second） and time（5s ,60s, 180s） , the two factors of three levels were combined out nine parameters and stimulated Neiguan by twisting manipulation, through many factor analysis of variance, the time, frequency and both the significant interaction were clear, by orthogonal design analysis, effect of different parameters on microcirculation was studied. Results ： The frequency has obvi- ous effect on the microcirculation, the influence of time and the interaction is weak. Input branch pipe diameter is the best combi- nation of fast frequency,medium time group （three times/s,60 seconds） ; output branch pipe diameter is the best combination of fast frequency,long time group （three times/s, 180 seconds）. Input/output the best combination of fast frequency, short time group（ three times/s,5 seconds）. It is concluded that in clinic acupuncture can improve circulation,increase the microcirculation perfusion with fast frequency, getting satisfactory results. Conclusion：