脾虚大鼠脑内TGF-β_1、TNF-α的变化以及益气扶正中药干预研究

Expression of transforming growth factor-β_1、tumor necrosis factor-α and interventional mechanism of Chinese medicinal with actions of replenishing qi and reinforce the healthy qi in rat spleen-deficiency model

目的:研究脾虚模型大鼠脑内转化生长因子β1(TGF-β1)、肿瘤坏死因子α(TNF-α)的活性表达,以及扶正益气中药的干预作用。方法:40只大鼠随机分成4组,正常组、模型组、治疗1组(四君子汤组)、治疗:2组(玉屏风散组),每组各10只。采用苦降泻下、饮食失节加劳倦过度法建立大鼠脾虚模型,采用免疫组化法检测下丘脑腹侧核、海马CA1区的TGF-β1和TNF-α表达变化与四君子汤玉屏风散的治疗作用。结果:TGF-β1在海马CA1区和下丘脑腹侧核表达明显降低,TNF-αmRNA在海马CA1区和下丘脑腹侧核表达明显降低;与模型组比较,治疗1组和治疗2组TGF-β1水平及TNF-αmRNA表达水平显著上升。结论:益气扶正中药四君子汤、玉屏风散可能通过影响免疫器官、调控细胞因子TGF-β1和TNF-α活性表达而调节机体免疫功能。

Objective：To study the changes of expressions of transforming growth factor-β1,tumor necrosis factor-α in spleen- deficiency rat model, and the interventional effects of Chinese medicinal with actions of replenishing qi and reinforce the healthy qi. Methods ： All rats （ n = 40 ） were randomly divided into the normal group, model group, treatment group 1 （ T group 1, treated with Sijunzi Decoction） and treatment group 2 （T group 2, treated with Yupingfeng Powder, eachn = 10 ）. The spleen-deficiency model was established in rats by using the methods of purgation ,improper diet and over strain. The expressions of TGF-β1 ,TNF- α in the ventral nucleus of hypothalamus and CA1 zone of hippocampus, and therapeutic effects of Sijunzi Decoction and Yupingfeng Powder were detected by applying immunohistochemistrlcal assay and in situ hybridization. Results：In the model group the TGF-β1 ,TNF-α expression decreased significantly; Compared with model group, TGF-β1 ,TNF-α expressions of treatment groupl and group 2 increased significantly in the ventral nucleus of hypothalamus and CA1 zone of hippocampus. Conclusion：Sijunzi Decoction and Yupingfeng Powder, with the actions of replenishing qi and reinforce the heathy qi, can regulate the immunologic function of the body through influencing immune organs and controlling the expressions of brain TGF-β1 and TNF-α.