人肝癌细胞中原癌基因Pokemon上调Shp2的表达

Proto-oncogene Pokemon upregulate the expression of Shp2 in human liver cancer

目的研究人肝癌细胞中Pokemon对Shp2表达的影响。方法通过RT-PCR、Real time PCR和Western blot方法检测人肝癌细胞系QGY-7703和人正常肝细胞系HL-7702中Pokemon和Shp2的mRNA和蛋白表达水平差异;并通过瞬时转染si-Pokemon RNA沉默QGY-7703细胞中Pokemon使其低表达,再检测Shp2的mRNA和蛋白水平变化。结果 Pokemon在人肝癌细胞系QGY-7703中的表达水平高于人正常肝细胞系HL-7702,Shp2的表达水平高低与Pokemon表达一致;在QGY-7703细胞中沉默Pokemon表达之后,Shp2的表达也相应降低。结论原癌基因Pokemon作为一种转录因子,可能调控Shp2的表达。

Objective To investigate the effect of Pokemon on expression of Shp2.Methods Real time PCR and Western blot were performed to measure mRNA level and protein level of Pokemon and Shp2 in QGY-7703 cells and HL-7702 cells,respectively.To determine whether reducing Pokemon expression would decrease the level of Shp2,QGY-7703 cells were transfected with si-Pokemon RNA and negative control using Lipofectamine 2000.Results Both real time PCR and Western blot indicate that Pokemon and Shp2 were overexpressed in QGY-7703 cells.More importantly,Shp2 and Pokemon expression is correlated in human liver cancer cell.Conclusions Pokemon might prompt liver cancer progression through upregulating the expression of Shp2.