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cDNA末端快速扩增技术及其方法的改进 被引量：1

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摘要 cDNA末端快速扩增(RACE)技术可以快速扩增mRNA3'和5'末端,从而获得全长的cDNA序列,为分离和研究新的基因提供了一个快速简便的方法。从RACE的原理出发,综述RACE技术的优缺点,阐述RACE技术的改良,并对其应用现状和发展前景进行了分析。
作者 贺斌 黄兴奇 余腾琼 钟巧芳 王玲仙 张秀 程在全
机构地区 云南省农业科学院生物技术与种质资源研究所 云南农业大学
出处 《浙江农业科学》 2012年第9期1352-1357,共6页 Journal of Zhejiang Agricultural Sciences
基金 国家转基因专项项目(2009ZX08001-007B) 云南省科技攻关项目(2010BB004)
关键词 CDNA末端快速扩增 RACE原理 RACE的优化 TdT加尾 RLM-RACE
分类号 S513 [农业科学—作物学]
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参考文献40

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浙江农业科学
2012年 第9期

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