吸烟对VEGF的影响及其与COPD关系的研究

The study of the effect of smoking on vascular endothelial growth factor and the relationship with chronic obstructive pulmonary disease

目的通过测定不同人群血液中血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达量,初步探讨VEGF与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)的关系及吸烟对其表达的影响。方法选择COPD急性加重期患者19例、稳定期患者20例及肺功能正常对照者33例,依据吸烟及肺功能情况分为:①吸烟伴COPD急性加重期19例;②吸烟伴COPD稳定期20例;③吸烟不伴COPD组18例;④不吸烟不伴COPD组15例。采用酶联免疫吸附法检测受试者血液中VEGF的水平。结果吸烟不伴COPD组与不吸烟不伴COPD组比较,血液中VEGF表达增强;吸烟伴COPD组包括稳定期及急性加重期与不吸烟不伴COPD组比较,VEGF表达明显增强。血液中VEGF表达与FEV1呈明显负相关(P<0.05),与吸烟指数呈正相关性(P<0.05)。稳定期COPD患者较COPD急性加重期VEGF表达无明显差异(P>0.05)。结论吸烟以及轻度COPD患者血液中VEGF表达上调,VEGF的过度表达可能参与患者气道与血管重建和气流受限的过程。吸烟刺激VEGF表达增加,VEGF尚不能作为COPD急性加重的判断指标。

Objective To analysis the relations between chronic obstructive pulmonary disease （COPD）and vascular en- dothelial growth factor （VEGF） by measuring blood VEGF of different subjects. Methods We draw 19 patients with acute exacer- bation stage of COPD, 20 patients with stable COPD and 33 control subjects with normal lung function, divided into 4 group by lung function and smoking：（1）19 subjects with acute exacerbation stage of COPD and smoking;（2）20 subjects with stable stage of COPD and smoking; （3）18 smoking subjects but not with COPD; （415 non-smoking subjects and not with COPD . Using en- zyme-linked immunosorbent assays （ELISA） to measure the level of VEGF in blood. Results The level of VEGF in blood of smoking group with COPD 〈 including stable stage and acute stage 〉 and not with COPD higher than the group of non-smoking and not with COPD ;and there is negative relation between VEGF and FEV1 （P 〈0.05 ）, but positive with smoking index（ P 〈 0. 05 ）. There is no important difference between stable stage and acute exacerbation stage of COPD . Conclusion Smoking and mild COPD can elevate the expression of blood VEGF, which may involved in the restructure of airway and vascular, and the process of airflow limitation;but VEGF can not as the diagnostic indicators of the acute exacerbation stage of COPD.