急性脑出血并发SIRS及MODS患者血清细胞因子流式分析

Determination of serum levels of cytokine in patients with acute intracerebral hemorrhage(AICH)leading to system inflammatory response syndrome(SIRS) which cause the multiple organ dysfunction syndrome(MODS) by flow cytometry

目的研究TNF-α、IL-10和IL-8在急性脑出血并发SIRS及MODS中的作用。方法按照SIRS诊断标准和MODS病情分期及严重程度评分标准,收集59例AICH诱发SIRS、导致MODS的患者,并将其分为单纯AICH组、AICH诱发SIRS组和AICH诱发SIRS致MODS组。利用CBA流式蛋白分析系统检测59例AICH诱发SIRS、导致MODS的患者血清TNF-α、IL-10和IL-8含量。结果单纯AICH组、AICH诱发SIRS组和AICH诱发SIRS致MODS组血清TNF-α、IL-10和IL-8水平全部高于正常对照组(P<0.05);AICH诱发SIRS和AICH诱发SIRS致MODS组血清TNF-α、IL-10和IL-8水平均显著高于单纯AICH组(P<0.05)。结论 TNF、IL-10和IL-8参与了AICH诱发SIRS致MODS的病理生理过程,在脑出血并发SIRS及MODS中的起重要作用。

Objective To study the role of the TNF-α,IL-10 and IL-8 play in the pathogenesis of acute intracerebral hemorrhage（AICH） leading to system inflammatory response syndrome （SIRS） which cause the multiple organ dysfunction syn- drome （MODS）. Methods According to the diagnosis standard of SIRS and MODS, 59 patients with acute introcerehral bomor- rhage were harvested, and were divided into 3 groups ： simple acute intracerebral hemorrhage （AICH） group, acute intracerebral hemorrhage accompanied with SIRS（AICH ＋ SIRS） group and acute intracerebral hemorrhage accompanied with MODS（ AICH ＋ SIRS ＋ MODS）group. The concentrations of TNF-α,IL-10 and IL-8 in serum of patients were analyzed by flow cytometry. Results The concentrations of TNF-α,IL-10 and IL-8 were significantly higher in all 3 groups than in normal group（ P 〈0. 05 ）. Signifi- cant differences between the concentrations of TNF-α,IL-10 and IL-8 in AICH group and those in AICH ＋ SIRS group were noted （P 〈 0. 05 ）. There was statistically difference in the concentrations of TNF-α, IL-10 and IL-8 between AICH group and AICH ＋ SIRS ＋ MODS group （P 〈 0.05 ）. Conclusion The TNF ,IL-10 and IL-8 may play an important role in the pathogenesis of AICH leading to SIRS which cause the MODS.