肺炎支原体肺炎IL-2、sIL-2R、IL-6、IL-8检测

Dynamic changes of IL-2,sIL-2R,IL-6and IL-8 in children with mycoplasma pneumonia and their clinical significance

目的探讨肺炎支原体肺炎(MPP)急性期和恢复期免疫功能动态变化及临床意义。方法检测IL-2、sIL-2R、IL-6、IL-8均采用ELISA双抗体夹心法。结果与对照组比较,MPP患儿急性期sIL-2R、IL-6、IL-8值均明显升高(P<0.01),IL-2值均明显下降(P<0.01),与急性期比较,MPP患儿恢复期IL-2值均明显升高(P<0.01),而sIL-2R、IL-6、IL-8值均明显下降(P<0.01);与对照组比较,MPP患儿恢复期IL-2、sIL-2R、IL-6、IL-8均无明显改变(P>0.05)。结论MPP患儿存在细胞免疫功能低下及紊乱,IL-2、sIL-2R、IL-6及IL-8值恢复较快,检测MPP患儿免疫功能,特别是IL-2、sIL-2R、IL-6和IL-8水平对疗效及预后的判定有重要价值。

Objective To observe the dynamic changes of IL-2, sIL-2R, IL-6 and IL-8 in children with mycoplasma pneumonia（MPP） and to study their clinical significance. Methods The serum levels of IL-2, sIL-2R, IL-6 and IL-8 in 40 chil- dren with MPP during the acute stage and convalescent stage were examined with double-antibody sandwich enzyme-linked immu- nosobent assay, and the results were compared with those in 30 normal children. Results The levels of sIL-2R, IL-6 and IL-8 were higher and IL-2 was lower during acute stage than those in the normal subjects （P 〈 0. 01 ）. Compared those during the con- valeseent stage, the level of IL-2was higher and sIL-2R, IL-6 and IL-8 were lower （P 〈0. 01 ） during the acute stage. There were not any significant differences of the levels of these four substances between the normal children and MPP children during the con- valescent stage. Conclusion The cellular immune function of children with MPP is impaired and the changes of IL-2, sIL-2R, IL-6 and IL-8 can be used to evaluate the curative effects and prognosis of MPP in children.