半边莲生物碱粗提物对骨髓瘤细胞U266的影响

Extract of alkaloids from Lobelia chinensis Lour and their inhibitory effects on growth of Myeloma Cells U266

目的探讨半边莲生物碱对骨髓瘤细胞U266体外增殖的影响。方法采用溶剂萃取对半边莲中的总生物碱进行了粗提取和纯化;采用细胞排染法、MTT比色法研究半边莲生物碱对U266细胞的体外增殖的影响;采用DNA凝胶电泳检测半边莲生物碱对细胞凋亡的影响。结果台盼蓝染色法和MTT结果表明半边莲生物碱对U266细胞有明显的抑制作用,且呈现浓度依赖效应;DNA凝胶电泳结果出现明显的梯形条带。结论半边莲生物碱能抑制骨髓瘤细胞U266的体外增殖,其作用机制可能是通过促进细胞发生凋亡。

OBJECTIVE To investigate the effects of alkaloids from Lobelia chinensis Lour on the growth of Myeloma cells U266.METHODS Rough alkaloids were extracted from Lobelia chinensis Lour by solvent extraction;their inhibitory effects on the growth of four different kinds leukemia cells were tested by MTT and Trypan blue staining;the influence of U266 cells′ apoptosis was detected by DNA agarose gel electrophoresis RESULTS The alkaloids of Lobelia chinensis Lour had the inhibitory effects on the growth of all kinds of leukemia cells,and the inhibition aggravated with the increase of alkaloids concentration;and DNA gel electrophoresis results showed obvious ladder.CONCLUSION The alkaloids of Lobelia chinensis Lour had the inhibitory effects on the growth of U266 cells,and would be provided with favorable exploitation perspective.