高效液相色谱法测定人血清中万古霉素浓度

Determination of vancomycin in patient′s serum by HPLC

目的建立一种快速测定人血清中万古霉素浓度的RP-HPLC法。方法采用液-液萃取法对血样进行预处理,以去甲万古霉素为内标。色谱柱：依利特C18柱（4.6mm×250mm,5μm）。流动相：乙腈-0.05mmol.mL-1磷酸二氢钾缓冲液（10：94,pH=3.2）,流速为1.0mL.min-1,紫外检测波长为230 nm。结果万古霉素血清浓度在5.00~100.00mg.L-1范围内呈良好的线性关系,（n=5,r=0.9996）,提取回收率〉95%,日内、日间RSD均小于10%。结论该方法方便、经济、无杂质干扰,适用于万古霉素血药浓度监测及药动学研究。

OBJECTIVE To establish a RP-HPLC method for determination of vancomycin in patient′s serum.METHODS The plasma samples were pretreated by liquid-liquid extraction.norvancomycin was treated as an internal standard,RP-HPLC was used with hypersil C18 column（250mm×4.5mm,5μm）,and acetoneitrile-0.05mmol·mL-1 potassium dihydrogen phosphate（0.05mmol·mL-1） buffer（10∶94,pH=3.2） was used as the mobile phase.The detection wavelength was 230 nm and the flow rate was 1.0mL·min-1.RESULTS The concentration of vancomycin in patient′s serum was linear at 5.00～100.00mg·L-1（n=5,r=0.9996）,The relative recoveries were higher than 95%.The RSD values of intra-day and inter-day were lower than 10%.CONCLUSION The method is accurate,sensitive,specific,economic,and reliable for vancomycin.It is suitable for therapeuticdrug monitoring and the pharmacokinetic study of vancomycin.