乳腺癌患者肿瘤细胞NGAL水平免疫组织化学测定的临床意义

Clinical Significance of Determination in Tumor Cells NGAL Levels of Breast Cancer Patients with Immuno-histochemistry

目的:探讨浸润性乳腺癌组织中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associatedlipocalin,NGAL)表达水平测定的临床意义。方法:60例乳腺癌患者及52例乳腺增生症患者分成两组,肿瘤细胞NGAL水平用免疫组化法检测并作分析。结果:①浸润性乳腺癌组织中的NGAL阳性表达率明显高于乳腺增生症;且前者为强阳性表达,后者阳性程度明显呈弱势,为阳性或弱阳性表达,阳性表达率分别为78.33.%和17.30%,两组之间阳性表达率比较存在显著性统计学差异(χ2=54.648,P<0.05)。②浸润性乳腺癌组织NGAL阳性表达率与病理进展关系统计数据显示,肿瘤直径<2cm与>2cm两组比较,NGAL阳性表达率无显著差异(χ2=2.103,P>0.05);有淋巴结转移组与无淋巴结转移组间比较NGAL阳性表达率差异有显著性(χ2=9.105,P<0.05),Ⅰ-Ⅱ级与Ⅲ-Ⅳ级组间阳性表达率差异也有显著性(χ2=11.211,P<0.05)。结论:浸润性乳腺癌组织中的NGAL阳性表达率与肿瘤直径大小关系不大,而与肿瘤的转移与否和分级呈平行相关。

Objective To explore the changes of expression in tumor cells NGAL levels of breast cancer patients and clinical significance of determination.Methods 60 patients with breast cancer patients,52 patients with mammary gland hyperplasia were divided into 2 groups.Their tumor cells NGAL levels were determined by immuno-histochemistry method.The data was processed and analyzed.Results The changes of the positive expression rate in tumor cells NGAL levels were significant higer of breast cancer patients than those in the patients with mammary gland hyperplasia（P 2cm between the two groups showed no significant difference（P〉0.05）.Tumor lymph node metastasis and without lymph node metastasis between the two groups compared showed significant difference（P〈0.05）.Grade Ⅰ-Ⅱand Ⅲ-Ⅳlevel group between the two groups compared with positive expression of a significant difference（P〈0.05）.Conclusion The changes of expression in tumor cells NGAL levels of breast cancer patients were related with the development of breast cancer and no significant relationship with tumor size.At the same time,also shows the correlation existed between the changes of the positive expression rate with tumor invasion and metastasis.