周期性张应力对人牙周膜细胞生物学活性的影响

Effect of cyclic stretch on human periodontal ligament fibroblasts activity

目的:探讨周期性张应力对体外培养的人牙周膜细胞生物活性的影响。方法:对体外培养的人牙周膜细胞施加不同大小的周期性牵张力(6%、12%、20%细胞表面拉伸率),24h后检测其对细胞的总蛋白合成、ALP活性、Col-Ⅰ和OCN分泌的影响。实验结果用SPSS 13.0进行统计分析。结果:较小的牵张力对人牙周膜细胞生物活性无显著影响,随力值的增大,牵张力能够呈强度依赖性抑制人牙周膜细胞的活性,减少总蛋白合成及ALP活性,抑制Col-Ⅰ及OCN的分泌。结论:周期性张应力可以抑制人牙周膜细胞的生物学活性,并呈强度依赖性。

Aim： To investigate the effect of cyclic stretch on human periodontal ligament fibroblasts activity in vitro. Method： Cyclic stretch of different magnitude （6%,12%,20%cell surface elongation） was forcedon human periodontal ligament fibroblasts in vitro for 24 hours, then we investigated cell total protein synthesis, alkaline phosphatase activities, collagen- Ⅰ and osteocalcin secretion. Data was analyzed with SPSS 13.0 soft ware. Results： The results showed that cyclic stretch of low intensity had no effect on biological activity of human periodontal ligament fibroblasts, but cyclic stretch of high intensity could inhibit total protein synthesis, alkaline phosphatase activities, collagen- Ⅰ and osteocalcin secretion of human periodontal ligament fibroblast significantly in a magnitude-dependent manner. Conclusion： Cyclic stretch could inhibit biological activity of human oeriodontal ligament fibroblasts in vitro in a mamaitude-deoendent manner.