老年心肌梗死与载脂蛋白E基因多态性相关性研究

Association of geriatric myocardial infarction and apolipoprotein E gene polymorphism

目的 研究老年心肌梗死 (MI)与载脂蛋白E(ApoE)基因多态性的相关关系以及ApoE对血脂水平的影响。 方法 应用聚合酶链反应—限制性片段长度多态性 (PCR RFLP)方法 ,测定 93例老年MI患者和 10 9例对照者的ApoE基因型 ;血脂水平按常规方法测定。 结果 本研究发现 4种ApoE基因型 :E 3/3、E 3/2、E 4/3及E 4/2。老年MI组ApoE 4/3基因型和ε4等位基因频率均显著高于对照组 (P <0 0 1) ;老年MI组总胆固醇 (TC)、低密度脂蛋白胆固醇 (LDL C)显著高于对照组 (P <0 0 1) ;在老年MI组ApoE基因各亚型之间 ,TC和LDL C水平之间存在统计学差异 (P <0 0 5 )。 结论 ApoE基因多态性与老年MI的发生发展有关并影响血脂的代谢水平 ;ε4等位基因可能是老年MI重要的遗传易患因素之一。

Objective To explore the relationship between geriatric myocardial infarction(MI)and apolipoprotein E(Apo E)gene polymorphism and to evaluate the effect of Apo E gene polymorphism on plasma lipid levels. Methods By using PCR-RFLP,we determined Apo E genotypes in 93 geriatric MI patients and 109 normal subjects of control.Other plasma lipid levels were measured by routine method. Results 4 genotypes of Apo E,i.e.E 3/3,E 3/2,E 4/3 and E 4/2,were detected in our study.There were statistically significant differences in Apo E4/3 and ε4 frequencies between geriatric MI patients and control group( P <0 01).MI patients also had significantly higher plasma total cholesterol(TC)and lower density lipoprotein cholesterol(LDL-C)than their control( P <0 01).Among different Apo E genetypes of MI patients,there were significant difference in TC and LDL-C level( P <0 05). Conclusions These results suggest that Apo E gene polymorphism is involved in the occurrence and the development of geriatric MI and also affects plasma lipid levels.Apo E ε4 might be an important genetic factor of geriatric MI.