匹马菌素的生物合成研究进展

Proceedings of pimaricin biosynthesis

匹马菌素是纳塔链霉菌产生的一种安全、高效的多烯大环类真菌抑制剂,广泛应用于医药领域与食品工业。作为26元环的糖基化多烯大环聚酮,其生物合成基因簇长度约110kb,包含19个基因,编码5个聚酮合酶、10个修饰和转运蛋白及4个调控因子。本文分析了匹马菌素生物合成的基因基础、多烯聚酮合成过程、氧化和糖基化修饰与调控机理等最新研究进展,展望了利用组合生物合成进行基因簇改造的方案与应用前景。

Polyene marcolide pimaricin produced by Streptomyces natalensis is a safe and efficient antifungal antibiotic and widely used in the disease treatment and food industry. Pimaricin has 26 carbon-ring with glycosylation, its biosynthesis gene cluster is approximately ll0kb , containing 19 genes encoding 5 polyketide synthases,10 modifying proteins and transporters, and 4 regulators. This article reviewed the recent proceedings and achievements on the process of polyene macrocyclic polyketide biosynthesis, oxygenate and glycosilation and regulation mechanisms during pimaricin biosynthesis The strategy of the combinatorial biosynthesis to modify the pimaricin biosynthesis gene cluster and the perspective applications were discussed.