放线菌HCCB01128的活性代谢产物及菌株鉴定

Bioactive metabolite and taxonomic identification of actinomycete strain HCCB01128

目的研究放线菌HCCB01128产生的抗肿瘤活性次级代谢产物。方法利用系统发育、形态特征和生理生化特性分析的方法对菌株HCCB01128进行初步鉴定。大孔吸附树脂吸附HCCB01128发酵液,反相色谱对菌株次级代谢产物进行分离和纯化。通过质谱、核磁共振氢谱(1H)、碳谱(13C)等分析对化合物1128-1进行结构鉴定。MTT法检测HCCB01128代谢粗提物的肿瘤细胞毒性。结果与结论菌株HCCB01128的16S rRNA与淀粉酶产色链霉菌Streptomyces diastatochromogenes的16S rRNA具有99.51%的相似性。其发酵初提物具有较强的抗肿瘤活性;经纯化鉴定其中一种活性化合物为Aurodox。本文首次报道从淀粉酶产色链霉菌中分离到Aurodox。

Objective To study the metabolites of stain HCCB01128. Methods The strain HCCB01128 was preliminarily identified to species level by phylogenetic analysis combined with morphological characteristics, physiological and biochemical characteristics. The metabolites from the culture broth of HCCB01128 were extracted by macroporous resin and purified by HPLC. The structure of compound 1128-1 was determined by IH and ~3C NMR analysis. The antitumor activities of the crude extract and compound 1128-1 were detected by MTT method. Results and Conclusion The 16S rRNA of strain HCCB01128 shared 99.51% similarity to Streptomyces diastatochromogenes. Compound 1128 showed apparent activities against tumor cells, and was identified as Aurodox. To our knowledge, this is firstly reported on isolation of Aurodox from Streptomyces diastatochromogenes.