一测多评法与外标法测定双黄连制剂中黄酮类成分含量的比较分析

Simultaneous Determination of Flavones in Shuanghuanglian Preparations by both QAMS and External Standard Method

目的分别采用一测多评法(QAMS)与外标法(ESM)测定双黄连制剂中4种黄酮类成分的含量,以证明一测多评法在复方中应用的可靠性。方法采用RP-HPLC,色谱柱:XtimateTMC18(4.6 mm×250 mm,5μm),流动相:甲醇(A)-0.2%磷酸水溶液(B),梯度洗脱,流速1 mL·min-1,柱温30℃,检测波长274 nm。结果一测多评法的计算值和外标法的实测值无显著差异。结论 "一测多评"法可作为一个新的质量评价模式用于双黄连制剂中黄酮类成分的质量评价。

OBJECTIVE To study the feasibility of quantitative analysis of multi-components by single-marker （QAMS） in determination of flavones in Shuanghuanglian preparations by comparing the results assayed by QAMS and external standard method（ESM）. METHODS The analysis was performed on a XtimateTM Cjs column （4. 6 mm ×250 mm,5μm）with methanol and water containing 0. 2% phosphoric acid as the mobile phase in gradient elution. The flow rate was set at 1.0 mL ·min^-1. The UV detector wavelength was 274 nm, and the column temperature was 30 ℃. RESULTS Within the linear ranges, the analysis results showed no significant difference between QAMS and ESM. CONCLUSION The QAMS method is credible and will be a new quality evaluation pattern for the determination of multiple flavones in Shuanghuanglian preparations.