微生物源性抗氧化剂对小鼠抗氧化性能及免疫功能的影响

Effects of Micro-derived Antioxidant on Antioxidant Ability and Immune Functions in Mice

旨在探究不同剂量的微生物源性抗氧化剂对小鼠抗氧化性能及免疫功能的影响。将60只28日龄雌性昆明小鼠随机分为4组,每组15只。试验组分别以0.5 g/kg bw*d(低)、1.0 g/kg bw*d(中)、1.5 g/kg bw*d(高)的剂量灌喂微生物源性抗氧化剂,每天1次,连续30 d,灌胃容量为0.2 mL/10 g bw*d,对照组灌喂等容量的蒸馏水。30 d后,测定微生物源性抗氧化剂对小鼠抗氧化性能及免疫功能的影响。结果表明,1.0 g/kg bw*d的微生物源性抗氧化剂可以极显著地提高血清中GSH-Px的活力(P<0.01),显著提高T-SOD活力(P<0.05),降低MDA的含量(P>0.05)。而1.5g/kg bw*d的微生物源性抗氧化剂可以极显著地提高血清中IgA的含量(P<0.01)和IL-2水平,以及ConA刺激的脾淋巴细胞转化率(P<0.01),促进脾脏和胸腺的发育(P>0.05)。提示1.0g/kg bw*d的微生物源性抗氧化剂可以显著增强小鼠的抗氧化能力,1.5 g/kg bw*d的微生物源性抗氧化剂可以增强免疫功能。

It was to investigate the effect of different doses of microbe-derived antioxidant on antioxidant ability and immune function in mice, sixty 28-day-old Kunming female mice with similar body weight are randomly divided into 4 groups, 15 mice of each group. The control group was fed basal diet, and the exper/mental groups were fed microbe-derived antioxidant with the dose of 0.5 g/kg bw＊d （ L-MA ）, 1.0 g/kg bw＊d （ M-MA ） and 1.5 g/kg bw＊d （ H-MA ） on the basis of basal diet for once a day, respectively. The experiment period was 30 days. The result showed that 1.0 g/kg bw＊d of microbe-derived antioxidant increased the enzyme activity of GSH-Px （ P〈0.01 ） and T-SOD （ P〈0.05 ） in serum of mice, and reduced the content of MDA （ P〉0.05 ） . While 1.5 g/kg bw＊d increased the content of IgA （ P〈0.01 ） and the level of IL-2 （ P〈0.01 ）, lymphocyte proliferation by ConA （ P〈0.01 ）, and promoted the development of spleen and thymus with no significant difference （ P〉0.05 ） . The result suggested that supplement of 1.0 g/kg bw＊d of microbe-derived antioxidant enhanced antioxidant ability of mice, while 1.5 g/kg bw＊d microbe-derived antioxidant enhance immune functions.