检测生精过程中减数分裂完成的质粒载体的构建

Construction of the Plasmid Vector Which Can Detect the Completion of Meiosis

旨在构建在所有转染细胞中表达绿色荧光、在核分裂的次级精母细胞、圆形精子细胞及长形精子细胞中表达红色荧光蛋白的cmv-eGFP-Gsg2-DsRed载体。重组载体是以本实验室保存CMV-EGFP-pDsRed质粒载体为基础载体,在其多克隆位点插入Gsg2启动子,期待可以启动红色荧光蛋白基因的表达。经大鼠睾丸共培养细胞转染试验证实,成功构建了含有Gsg2启动子的表达双荧光蛋白质粒载体。

It was to intend to construct cmv-eGFP-Gsg2-DsRed vector which can start the expression of EGFP in all transgenic cells and start the expression of DsRed in the germ cells that have completed the meiosis proceed. The recombinant vector is based on the CMV-EGFP- pDsRed vector which is conserved in our laboratory, and the Gsg2 promoter is inserted into the MCS. The transfectian test to the rat testis co- culture cells, suggest that cmv-eGFP-Gsg2-DsRed vector which contains the Gsg2 promoter is successfully constructed.