忍冬种质资源遗传多样性的AFLP分析

Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis

目的:研究国内主产区忍冬种质资源的遗传多样性,探讨它们之间的亲缘关系,并为新品种选育提供参考。方法:选用6对引物组合对13个忍冬种质进行AFLP分析,利用DPS V3.01软件计算各品种的遗传距离,按UPGMA法进行聚类分析。结果:共获得435条扩增带,其中191条具有多态性,平均多态检出率为43.9%。聚类结果表明,同一道地产区的栽培品种亲缘关系较近,山东种质基于AFLP标记的分类结果与形态特征基本一致。结论:AFLP分子标记揭示出忍冬种内存在丰富的遗传多样性,为忍冬种质资源的合理利用及品种选育提供了分子水平的理论依据。

Objective： This study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica. Method： Using 6 primer combinations, 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software, and the cluster was conducted based on UPGMA. Result： A total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cuhivated variety from the same genuine area was near, and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character. Conclusion： AFLP marker can indicate the abundant ge- netic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in mo- lecular level.