灵芝多糖对高脂性脂肪肝大鼠氧化应激的影响

Effect of Ganoderma lucidum polysaccharides on oxidative stress of hyperlipidemic fatty liver in rats

目的:研究灵芝多糖对高脂性脂肪肝大鼠氧化应激的影响。方法:SD大鼠72只,随机分为6组:正常对照组(N组),模型组(M组),灵芝多糖低(GLPs-L)、中(GLPs-M)、高(GLPs-H)剂量组(200,400,800 mg·kg-1)和辛伐他汀组(SV 1.8mg·kg-1)。用高脂饲料喂养建立大鼠高脂性脂肪肝(HFL)模型。经12周给药后,分别测定各实验组血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和总抗氧化能力(T-AOC)以及肝组织中MDA,SOD,GSH-Px和T-AOC的含量;光镜观察肝组织脂肪病变程度。结果:M组血清TC,TG,LDL-C含量显著升高(P<0.01);与M组比较,GLPs-M,GLPs-H组血清TC,TG,LDL-C含量均显著降低(P<0.05或P<0.01),GLPs-H组HDL-C含量显著增加(P<0.05);GLPs-M,GLPs-H组血清和肝匀浆中MDA含量较M组相比均明显降低(P<0.05或P<0.01),SOD,GSH-Px和T-AOC水平较M组相比均明显上升(P<0.05或P<0.01)。GLPs-M,GLPs-H组可使肝细胞脂肪变性程度明显减轻。结论:灵芝多糖能明显降低高脂性脂肪肝大鼠血脂水平,有效对抗氧化应激,对大鼠高脂性脂肪肝有一定的防治作用。

Objective ： To study the effect of Ganoderma lucidum polysaccharides on oxidative stress of hyperlipidemic fatty liv- er in rats. Method： Seventy-two SD rats were randomly divided into six groups, namely the normal control group （NG） , the model group （MG）, the G. lucidurn polysaceharides groups of low, middle and high dose （GLPs-LG, GLPs-MG, GLPs-HG） and the Simv- astatin group （SV）. The rats were fed with high fat diet to establish the model of hyperlipidemie fatty liver in rats. After administration for 12 weeks, rats in each group were tested with the following indexes： total cholesterol （TC）, triglyceride （TG）, high density lipo- protein-cholesterol （ HDL-C ） and low density lipoprotein-cholesterol （ LDL-C ） , malondialdehyde （ MDA ） , superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and total antioxidant capacity （T-AOC） in serum as well as the contents of SOD, MDA, GSH-Px and T-AOC in hepatic tissues. Histopathological changes of hepatic tissues were observed under light glass. Result ： The con- tents of TC, TG and LDL-C were significantly increased in the model group （ P 〈 0. 01 ）. Compared with the model group, both the GLPs-M group and the GLPs-H group showed significant decreases in TC, TG and LDL-C （ P 〈 0. 05 or P 〈 0. 01 ）, while the GLPs-H group showed a notable increase in HDL-C （ P 〈 0.05 ）. Compared with the model group, both the GLPs-M group and the GLPs-H group showed significant decreases in MDA （ P 〈 0. 05 or P 〈 0. 01 ） and notable increases in SOD, GSH-Px, T-AOC （ P 〈 0.05 or P 〈 0. 01 ）. The GLPs-M group and the GLPs-H group proved a remarkable alleviation in fatty degeneration of hepatic cells. Conclu- sion： G. lucidum polysaccharides can significantly reduce the blood fat level of hyperlipidemic fatty liver in rats and effectively inhibit oxidant stress, showing the effect on preventing and treating hyperlipidemie fatty liver in rats to some extent.