利用流式细胞术研究榄香烯对肝癌腹水瘤细胞系Hca-F25/CL-16A3的抗肿瘤作用

The study of the antitumor activity of elemene by flow cytometry(FCM)

采用小鼠肝癌腹水瘤细胞系Hca F2 5/CL 16A3,利用流式细胞术 (Flowcytometry ,FCM)研究了榄香烯对肿瘤细胞的作用、对Hca F2 5/CL 16A3细胞周期的影响、DNA含量的分析以诱导细胞凋亡。结果表明 :榄香烯可以明显抑制Hca F2 5/CL 16A3细胞的增殖 ,有效地杀伤肿瘤细胞 ;榄香烯对GO/G1期细胞无明显作用 ,主要作用于S期 ,阻止由S期进入G2 /M期 ,从而抑制肿瘤生长 ;可在GO/G1期前出现一个凋亡特异的AP峰。

In the review,author analyzed the antitumor activity of elemene preparation,influence for Hca F25/CL 16A3,DNA content and the function with induction apoptosis with Hca F25/CL 16A3 by flow cytometry(FCM).Result:Elemene could inhibit the growth of tumor abviously,kill the tumor cell.During period of the reaction,GO/G1phase did not change,elemene could arrest cell cycle from S phase to G2/M phase transtion and make it more in S phase.At last,inhibit the tumor cell,and become a AP phase in front of GO/G1phase. [WT5HZ]