栓线长度、直径及大鼠体重与栓线法大鼠局灶性脑缺血模型关系的研究

The relation of rat MCAO model quality to thread diameter,length and body weight of the rats

目的 :为提高栓线法大鼠局灶性脑缺血 (MCAO)模型的成功率和稳定性 ,探讨大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。方法 :利用 2× 2× 2析因设计 ,通过检测神经功能缺陷评分和脑梗塞体积 ,分析大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。结果 :大鼠体重、栓线直径两水平之间有差异 (P <0 0 5) ,栓线插入长度两水平之间无差异(P <0 0 5) ;大鼠体重、栓线直径之间有正交互作用 (P <0 0 5) ,其余两两之间以及三者之间无交互作用 (P >0 0 5)。结论 :最佳造模方案是大鼠体重 2 50～ 30 0g、线长 17mm或 18mm、线直径 0 2 8mm ,该方案制作的MCAO模型成功率高 ,重复性好。

Objective:To clarify the relation of middle cerebral artery occlusion [MCAO(intraluminal thread method)]model to the filament diameter and length and rat weight.Methods:The relation of the filament diameter and length,rat weight was analyzed with 2×2×2 factorial design,via assessing neurlolgical deficit scores.Results:The neurological deficit scores betwwen the two levels of the rat weight or the fliament diameter were significantly different (P<0 05).No significant difference was found between the two levels of the filament length(P>0 05). A positive interaction was shown between the rat weight and the filament diameter(P<0 05),but no interaction among other factors.Conclu-sion:The reliability and reproducibilty of this MCAO model were greatly improved,when following the optimal method(rat weight 250g-300g,filament diameter 0 28 mm and filament length 17 mm or 18 mm. [WT5HZ]