人乳头瘤病毒与中国妇女宫颈癌相关性的研究(Ⅲ.子宫颈增殖性病变中人乳头瘤病毒16型基因组物理状态的分析)

Studies on Correlation between Human Papilloma Virus and Cervical Cancer of Womem in China(Ⅲ. Analysis on the Physical State of HPV—16 DNA in Cervical Proliferative Lesions)

本研究以a—^(32)P—dCTP标记的HPV—16DNA为探针,应用限制性内切酶技术和Southern印迹杂交方法进一步分析了32例HPV—16DNA斑点杂交阳性的宫颈活检标本中HPV—16基因组的物理状态。结果表明:正常宫颈上皮和宫颈良性增殖性病变(宫颈湿疣)中,HPV—16DNA均以染色体外游离基因的形式存在;宫颈癌前病变(宫颈上皮内新生物)中,HPV—16DNA以游离和整合两种状态共存;宫颈恶性增殖性病变(宫颈癌)中,HPV—16DNA完全以整合的形式存在于宿主细胞基因组中。以上结果提示:宫颈上皮中HPV—16基因组的整合在宫颈癌的发生发展过程中可能起着重要作用。

The Physical state of 32 cases of cervical proliferative lesions which were positive to dot blot hybridization of HPV—16DNA were further analyzed by using labelled HPV—16 DNA probe, restriction enzyme technique and Southern blot hybridization method. The results showed that HPV—16 DNA sequences could only be detected as an episome in normal cervical epithelia and cervical benign proliferative lesions(cervical condyloma),on the contray, HPV—16 DNA was found to have been integrated into the genome of host cell in of cervical malignant proliferative lesions(cervical cancer) while in the cervical malignant lesions(cervical intraepithelial neoplasia, CIN), HPV—16 DNA sequences coexisted in form of episome and integration. These results suggest that integration of HPV—16 genome in cervical epithelia may play an important role in the process of occurrence and development of cervical cancer.