端粒酶活性在人乳癌中的表达

Expression of Telomerase Activity In Human Bresat Cancer

目的 :比较端粒酶活性在乳腺良、恶性病变中的异同 ,探讨其在乳癌诊断中的意义和与普遍认定的预后指标间的关系。方法 :用端粒重复扩增 (TRAP)检测了 54例乳癌、44例乳腺良性病变组织的端粒酶活性。结果 :54例乳癌中 49例 ( 90 .74% )显示端粒酶活性 ,1例纤维腺瘤有弱端粒酶活性。端粒酶活性与肿瘤大小及分期有关 ,而与腋淋巴结转移及雌、孕激素受体之间无关。结论 :端粒酶活性见于绝大多数浸润性乳癌及极少数纤维瘤 ,检测该酶活性可能在乳癌的诊断及预后中发挥重要作用。

objective:To Compare telomerase activity in brest cancer with benign breast lesions,to analyze the significance of telomerase activity in diagnosis of breast cancer and to evaluate the possible associations with well-known prongnostic factors in breast cancer.Methods: Telomerase activity was detected in 54 cases of breast cancer and 44 cases of benign breast lesions using TRAP assay.Results:Telomerase activity was detected in 49 of 54(90.74%)cases of breast cancer.1 of 20 cases fibreadenoma were feebly positive for telomerase activity.A singnficant correlation was found between telomerase activity and tumor size and stage.No statistical assotiation was seen between enzyne activity and lymph node metastasis status.Conclusions:Telomerase activity is only detected in most invasive breast cancer and few cases of fibroadenoma,Telomerase detection by the TRAP method may be of vacue in dignosis and prognosis of breast cancer.