黑升麻异丙醇提取物对去卵巢大鼠中心体温的影响

Effects of an isopropanolic-aqueous black cohosh extract on central body temperature of ovariectomized rats

目的观察黑升麻异丙醇提取物(ICR)对去卵巢大鼠中心体温的影响。方法雌性SD大鼠分为4组:去卵巢(OVX)组、假手术组、去卵巢后戊酸雌二醇(OVX+E)组和去卵巢后ICR组,相应的药物治疗并连续监测体温6周。结果 4组大鼠中心体温均保持昼低夜高的正常生理节律。日间和夜间中心体温,OVX组、(OVX+E)组和(OVX+ICR)组均高于假手术组;日间中心体温,(OVX+E)组和(OVX+ICR)组均低于OVX组。假手术组的昼夜温差最小;而(OVX+E)组和(OVX+ICR)组的昼夜温差均较OVX组增大。日间体温波动幅度、夜间体温波动幅度以及体温波动较大次数:OVX组均较假手术组增高;(OVX+ICR)组夜间体温波动幅度,均较OVX组增高;(OVX+ICR)组日间体温波动幅度较OVX组低。结论大鼠去卵巢后可发生体温调节异常。黑升麻异丙醇提取物改变大鼠中心体温,且对去卵巢大鼠日间中心体温影响明显。

Objective To examine the effect of isopropanolic black cohosh extract（ICR） on the central body temperature（CBT） of ovariectomized rats（OVX） and elaborate its possible effects in alleviating menopause related hot flushes.Methods Female Sprague-Dawley rats were divided into four groups： ovariectomy（OVX）,sham,ovariectomy plus estradiol valerate（OVX＋E）,and ovariectomy plus ICR（OVX＋ICR）.A temperature implant was embedded in the abdominal cavity of all four groups.The temperature of all animals were monitored for 6 weeks.Results CBT of four groups maintained a normal circadian rhythm,with a low day CBT and a high night CBT.CBTs of the sham group were lower than that of the other three groups.The day CBTs of the（OVX＋E） group and（OVX＋ICR） group were lower than that of the OVX group.For the difference between day and night CBT,the sham group was smallest,while（OVX＋E） and（OVX＋ICR） groups were higher than that of OVX group.The amplitude of day CBT of（OVX＋ICR） group was lower than that of OVX group.Conclusion Ovariectomized rats had abnormal thermoregulation.The herbal extract ICR can affect abnormal thermoregulation and had a significant effect on day CBT of ovariectomized rats.