红花注射液溶血与凝聚试验的探讨

Study on hemolytic and blood cell agglutination test of safflower injection

目的建立红花注射液溶血与凝聚试验的检查法。方法采用《中国药典》2010年版溶血与凝聚检查法和分光光度法进行试验。试验组分别用0.1～1.1mL各制备3个平行样。阴性对照组用0.9%氯化钠溶液、阳性对照用蒸馏水。每个试验管内加2%的兔红细胞混悬液。结果在两种方法检测后,红花注射液不对兔红细胞产生溶血与凝聚现象。结论红花注射液可按《中国药典》规定的方法进行溶血与凝聚试验。

Objective To establish the method of hemolytic and blood cell agglutination test on safflower injection. Methods Hemolytic and blood cell agglutination test, spectrophotometer was used to test according to Chinese Pharmacopoeia 2010 edition. Experimental group were used 0.1 to 1.1 mL samples of the preparation of three parallel. The negative control group were used 0.9%NaCI while positive control group were used distilled water. 2% percent red blood cell suspension of rabbit was added to every sample. Results It was proved that safflower injection did not produce hemolytic and blood cell agglutination. Conclusion Safflower injection is prescribed by hemolytic and blood cell agglutination test.