CLEC4L在人胎盘组织及滋养层细胞的分布与定位

The distribution and location of CLEC4L in the human placental tissue and trophoblast cells

目的细胞间黏附分子非整合素蛋白3(human C-type lectin domain family 4,memberL,CLEC4L)为主要分布在树突状细胞(DC)表面的一个外源性凝集素超家族分子,通过依赖Ca2+的碳水化合物识别区域(CRD)形成寡聚糖,增强与抗原的结合能力。CLEC4L作为丙型肝炎病毒(HCV)受体,对HCV感染和传播起重要作用。本研究旨在明确人胎盘组织及胎盘滋养层细胞中CLEC4L的分布与定位。方法免疫组织化学方法检测CLEC4L在人滋养层细胞内定位以证明CLEC4L的存在,同时免疫组织化学单染及荧光双染法检测不同孕期正常胎盘组织CLEC4L定位与表达。结果人胎盘滋养层细胞中检测到CLEC4L表达,主要分布于滋养层细胞表面;同样在早孕期的绒毛及足月胎盘中均检出CLEC4L的表达,但在足月胎盘中的表达明显低于早孕期的绒毛组织。结论实验显示不同孕期胎盘组织均可见CLEC4L的表达,其在孕早期表达较高。滋养层细胞上该受体可能与HCV胎盘宫内感染有关。

Objective Human C-type lectin domain family 4, member L （CLEC4L） is a kind of exogenous lectin superfamily molecules mainly distributed on the surface of dendritic cell （DC）. CLEC4L can form Mannan oligosaccharides （MOS） through the Ca2＋-dependent carbohydrate recognition domain （CRD） , so as to enhance the antigen binding capacity. As a receptor of hepatitis C virus （HCV）, CLEC4L is also very important in the process of HCV infection and transmission. The present study was to determine the distribution and location of CLEC4L in the human placental tissue and trophoblast cells in vitro. Methods The distribution of CLEC4L in human trophoblast cells was determined by Immuno- histochemistry（IHC）. IHC and fluorescence double staining were done to prove the existence in different stages of placental tissue. Results CLEC4L was mainly distributed on cell membrane of placental trophoblast cells, and in human placenta, especially villi of first trimester. Conclusion CLEC4L was expressed in human placenta and trophoblast cells cultured in vitro, which was higher in the early stage of pregnancy. The study provides a cellular basis for experiment in vitro to investigate the mechanism of receptor mediated HCV intrauterine transmission.