十二烷基磺酸钠纳米胶团-细胞色素c自组装高效纳米结构过氧化物酶

A Self-Assembled Nano-Structrured Peroxidase Based on Sodium Dodecyl Sulfate Nano-Micelle and Cytochrome c

用阴离子表面活性剂十二烷基磺酸钠与细胞色素c自组装的方法构建了一种纳米超分子结构,观察到其具有显著的过氧化物酶活性,且在pH为10.5时达到最高。这种纳米结构过氧化物酶的催化效率为0.0219μmol/L.s。电化学方法测得其电子传递速率常数ks为0.586 s-1。这种以自组装方法构建的超分子结构不仅具有较高活性,可在天然过氧化物酶自杀性失活底物浓度较高时运用,且可固定化于电极上,实现与电极间的直接电子传递。

A nano-structured supermolecule or artificial enzyme was built self-assembly based on sodium dodecyl sulfate nano-micelle and cytochrome c. A significant peroxidase activity was observed and reached the maximum value at pH 10.5. Its catalytic efficiency was evaluated to be 0.0219 μmol/L.s. The immobilized nano-structured peroxidase modified electrode showed quasi-reversible electrochemical redox behaviors with a formal potential of （-38± 5） mV （vs. Ag/AgCI） at a scan rate of 0.05 V/s. The cathodic transfer coefficient and electron transfer rate constant were evaluated to be 0.51 and 0.59 s-1, respectively. The apparent Michaelis-Menten constant （Km^app） was calculated to be 0.14 mmol/L. These results suggest that the nano-structured peroxidase not only perform a high activity as peroxidase and can be used in high concentration of hydrogen peroxide, but also can be immobilized on the electrode and realize direct electrochemical behavior.