脑栓通对脑缺血再灌注大鼠神经血管单元的保护作用

Protective effects of Chinese herbal medicine Naoshuantong on neurovascular unit in rats with cerebral ischemia/reperfusion injury

目的:观察中药脑栓通对脑缺血再灌注大鼠神经血管单元主要成分神经元、星形胶质细胞以及脑血管内皮细胞的保护作用。方法:30只雄性Sprague-Dawley大鼠随机分为假手术组、缺血再灌注组和1g/(kg·d)脑栓通组。采用大脑中动脉线栓法建立局灶性脑缺血损伤模型,1.5h后拔出线栓实施再灌注。再灌注24h时后进行大鼠神经功能缺损评分,采用末端脱氧核苷酸转移酶介导的脱氧尿苷原位末端标记法结合神经元核抗原、胶质纤维酸性蛋白及CD31免疫荧光双染检测缺血再灌注24h时缺血灶周边的神经元、星形胶质细胞以及脑血管内皮细胞的凋亡情况。结果:脑栓通能显著改善脑缺血损伤大鼠的神经功能缺损,减少缺血灶周边星形胶质细胞、脑血管内皮细胞和神经元的凋亡。结论:脑栓通通过保护脑缺血再灌注后大鼠的神经血管单元促进神经功能的改善。

To investigate the protective effects of Naoshuantong, a compound traditional Chinese herbal medicine, on the main components of neurovascular unit in rats with cerebral ischemia/reperfusion injury. METHODS： A total of 30 male Sprague-Dawley rats were randomly divided into sham-operated, ischemia/ reperfusion, and ischemia/reperfusion plus Naoshuantong groups. The cerebral ischemia was induced by 1.5 h of middle cerebral artery occlusion, followed by unlocking the thread to induce reperfusion injury. After 24 h of reperfusion, neurological functional deficits were assessed, apoptosis of main components of neurovascular unit was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling combined with neuronal nuclei antigen, glial fibrillary acidic protein or CD31 immunofluorescence double staining. RESULTS： Naoshuantong significantly reduced neurological functional deficits, and reduced neuron, astrocyte and vascular endothelium cell apoptosis induced by ischemia/reperfusion injury in the border zone of ischemic cortex. CONCLUSION. Naoshuantong is effective in protecting the neurovascular unit including neurons, astrocytes and vascular endothelium cells against cerebral ischemia/reperfusion-induced apoptosis.