结缔组织生长因子在慢性肝病诊断中的应用研究

Diagnostic application of connective tissue growth factor in chronic liver diseases

探讨结缔组织生长因子(CTGF)在慢性肝病诊断中的应用价值。运用ELISA方法检测198例研究对象,观察血清CTGF水平的变化,分析CTGF水平与临床特征及实验室指标的相关性;运用实时荧光定量RT-PCR方法检测组织中CT-GF mRNA的表达量,比较各组间表达量的变化。乙肝后重度肝纤维化组、肝硬化组、原发性肝细胞癌(HCC)组的血清CTGF水平均显著高于正常对照组,且具有统计学意义(P<0.05);血清CTGF水平与部分临床实验室特征存在相关性,在区分早晚期肝纤维化上具有诊断提示作用;癌旁组织的CTGF mRNA相对表达量显著高于正常对照和癌组织的表达量且具有统计学差异(P<0.05)。血清CTGF水平与肝纤维化、肝硬化及肝癌的发生发展关系密切,有望成为一个新的无创性肝纤维化检测诊断指标,为临床诊断提供依据。

To investigate the diagnostic application value of connective tissue growth factor （CTGF） in chronic liver diseases, the serum CTGF level was detected in 198 cases by enzyme linked immunosorhent assay （ELISA）. The correlation between se rum CTGF levels and other clinical laboratory features was analyzed. The expression levels of CTGF mRNA was compared a mong various disease groups using RTPCR. The CTGF levels in severe hepatic fibrosis group, liver cirrhosis group and hepa tocellular carcinoma （HCC） group were significantly higher than that in the control group（P〈0.05） ;and the CTGF levels were correlated with some clinical laboratory features. Serum CTGF level would be a valuable alternative in distinguishing early or severe period liver fibrosis. The mRNA expression level of CTGF in corresponding adjacent liver tissues was higher than that in HCC and control group（P〈0.05）. Serum CTGF level is related to the pathological progress of liver fibrosis, cirrhosis and HCC.