OPN和VEGF-C在胃癌中的表达及其临床意义

Clinical signif icance of OPN and VEGF-C expression in gastric cancer

目的:评价骨桥蛋白(osteopontin,OPN)和血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)在胃癌中表达及与临床病理参数之间相关性,进一步探讨二者的共表达在胃癌淋巴结转移中的机制.方法:收集复旦大学附属中山医院手术切除胃癌组织标本及患者相关临床资料,对纳入研究的93例胃癌原发灶标本采用免疫组织化学染色法(EnVision二步法),检测OPN与VEGF-C蛋白的表达.结果:93例胃癌组织中OPN与VEGF-C的阳性表达率分别为64.5%(60/93)和69.9%(65/93),在非肿瘤性胃黏膜中均未见其阳性表达;OPN和VEGF-C的表达与胃癌浆膜侵犯、TNM分期以及淋巴结转移呈明显相关(P<0.05).蛋白之间的相关性分析显示,OPN与VEGF-C之间存在正相关(r=0.493,P<0.01).结论:联合检测OPN与VEGF-C有助于阐述胃癌发生发展、浸润转移的机制,OPN可能通过上调VEGF-C的表达促进胃癌的淋巴结转移.

AIM： To evaluate the expression of osteopontin （OPN） and vascular endothelial growth factor C （VEGF-C） in gastric cancer and to analyze their correlation with clinical pathological characteris- tics of gastric cancer. METHODS： Surgical specimens of gastric cancer and related clinical data were collected from the Affiliated Zhongshan Hospital of Fudan University. The expression of OPN and VEGF-C proteins in 93 gastric cancer specimens was ex- amined by immunohistochemistry. RESULTS： The positive rates of OPN and VEGF-C expression in 93 gastric cancer speci- mens were 64.5% （60/93） and 69.9% （65/93）, re- spectively. The expression of OPN and VEGF-Cwas significantly correlated with serosal inva- sion, tumor TNM stage, and lymph node me- tastasis （all P 〈 0.05）. Moreover, the expression of OPN was positively correlated with that of VEGF-C in gastric cancer （r = 0.493, P 〈 0.01）. CONCLUSION： OPN and VEGF-C protein expression might be associated with the de- velopment, invasion, and metastasis of gastric cancer. OPN may play a role in the lymph node metastasis of gastric cancer by up-regulating the expression of VEGF-C.