Tiam1和MMP-14在宫颈癌中表达的临床意义

Clinical significance of expression of Tiam1 and MMP-14 in cervical cancer

目的检测Tiam1和MMP-14在宫颈鳞癌、宫颈上皮内瘤变和正常宫颈中的表达,了解其在宫颈鳞癌的发生发展以及浸润转移方面的作用。方法收集某院病理科2010年6月～2012年6月手术切除宫颈鳞癌标本100例,宫颈上皮内瘤变30例,正常宫颈10例。采用免疫组化二步法检测两者在宫颈组织中的表达情况。采用SPSS18.0软件包进行统计分析。结果 Tiam1在宫颈鳞癌中的表达高于CIN和正常宫颈(P<0.01);Tiam1的表达与分化程度、深肌层浸润和淋巴结转移有关(P<0.05);MMP-14在宫颈鳞癌的表达高于CIN和正常组织,且在CIN的表达亦高于正常组织(P<0.05);MMP-14的表达与深肌层浸润和淋巴结转移有关(P<0.05);两者在宫颈鳞癌的表达强度呈正相关。结论 Tiam1与MMP-14可能都参与了宫颈鳞癌的恶性发展过程,且与宫颈癌的深肌层浸润、淋巴结转移密切相关。两蛋白可能在宫颈鳞癌的发生发展过程中具有协同作用,表达阳性的癌细胞可能具有更强的浸润转移的潜能。

Objective To detect Tiaml and MMP-14 expression in cervical squamous cell carcinoma,cervical intraepithelial neoplasia and normal cervix, understand in the development of cervical squamous cell carcinoma, as well as the role of the invasion and metastasis. Methods Conventional paraffin sections of 10 normal cervical tissues,30 cervical intraepithelial neoplasias and 100 cervical squamous cell carcinoma, collected from hospital from June 2010 to June 2012, were detected by immunohistochemical method （IHC） to assess the expression of Tiaml and MMP-14. SPSS18.0 package were used for all data analysis. Results Tiaml expression in cervical squamous cell carcinoma was higher than in CIN and normal cervix （P 〈 0.01 ） ;Tiaml expression was related to the degree of differentiation, deep myometrial invasion and lymph node metastasis （P 〈 0.05）; MMP-14 expression in cervical squamous cell carcinomahigher B was higher than that of CIN and normal tissue, and expression in CIN B was higher than normal tissue （P 〈 0.05） ;MMP-14 expression B related to deep myometrial invasion and lymph node metastasis （P 〈 0.05） ;both The expression intensity in cervical squamous cell was positively correlated. Conclusion Tiaml and MMP-14 may be involved in the malignant process of development of cervical squamous cell carcinoma and deep myometrial invasion and cervical lymph node metastasis. The two proteins may have a synergistic effect in the development and progression of cervical squamous cell carcinoma,the expression of positive cancer cells may have stronger potential invasion and metastasis.