青蒿素对大鼠心肌梗死后心肌纤维化的影响及其机制

The effect and mechanism of artemisinin on the myocardial fibrosis after myocardial infarction in rats

目的探讨青蒿素对大鼠心肌梗死后心肌纤维化的影响及其机制。方法成年SD大鼠随机分为假手术组和心肌梗死组。心肌梗死组分为青蒿素组和对照组,青蒿素组给予青蒿素治疗,按照75 mg/(kg.d)剂量灌胃,每日3次。对照组和假手术组灌胃等量的0.5%羧甲基纤维素钠溶液。所有给药都在结扎冠脉后24 h开始。结果四周后,与对照组比较,青蒿素组生存率增加、心功能显著改善(P<0.05),而且梗死周边区的心肌纤维化程度以及纤维化相关蛋白表达显著降低。结论青蒿素下调转化生长因子β1(TGF-β1)蛋白的表达,减少心肌纤维化,改善心功能。

Objective To explore the effect and mechanism of artemisinin on the myocardial fibrosis after myocardial infarction in rats.Methods Adult male Sprague Dawley rats were divided into sham group and MI group which were divided into artemisinin group and control group,the artemisinin group was treated with artemisinin of 75 mg/（kg·d） by gavage,3 times/day,the control group and sham group were treated with 0.5% of carboxymethyl cellulose equivalently.Each treatment was started after ligation of the left anterior descending coronary artery for 24 hours.Results Four weeks after,compared with the control group,the survival rate in artemisinin group increased,and the cardiac function improved significantly（P 0.05）.Moreover,the degree of myocardial fibrosis and elevated levels of fibrosis-related factors decreased remarkably.Conclusion Artemisinin can improve cardiac function and attenuate post-infarct myocardial fibrosis by down-regulating TGF-β1.