脐带间充质干细胞联合移植对胰岛活性与功能的影响

Co-transplantation of islets and umbilical cord mesenchymal stem cells improves graft activity and function in rats

目的:探讨脐带间充质干细胞(umbilical cord mesenchymal stem cells,UCMSCs)与胰岛细胞联合移植对胰岛活性与功能促进作用.方法:50只链脲佐菌素(streptozotocin,STZ)诱导的SD大鼠随机分为4组移植于肾包膜下,(1)500个胰岛移植组;(2)1×106个UCMSCs与500个胰岛细胞联合移植组;(3)1×106个UCMSCs组;(4)假手术(空白)对照组,仅于肾包膜下注入0.3mL生理盐水;术后测血糖水平、葡萄糖耐量试验(glucose tolerance test,GTT)、血清胰岛素水平以及达正常血糖水平率(以血糖浓度≤11.1mmol/L标准为正常)至28d.结果:联合移植组术后血糖水平最低,血清胰岛素水平更高,达正常血糖浓度率优于其他3组,在术后14d GTT较其他3组好,血清胰岛素水平较其他3组好,血糖水平、血清胰岛素水平、GTT水平UCMSCs组与对照组间均无统计学差异,单纯UCMSCs组无1例动物血糖水平达正常.结论:间充质干细胞与胰岛联合移植能有效提高胰岛细胞活性与功能.

AIM：To examine the effect of co-transplantation of islets and umbilical cord mesenchymal stem cells（UCMSCs） on graft activity and function in rats.METHODS：Streptozotocin-induced diabetic Sprague-Dawley（SD） rats were divided into four groups,with（1） 500 islets,（2） 1 to 5×106 UCMSCs,（3） 500 islets and 1 to 5×106 UCMSCs;and（4） normal saline transplanted under the kidney capsule,respectively.All rats were evaluated for blood glucose,serum insulin,glucose tolerance,and the rate of normoglycaemia（blood glucose ≤ 11.1 mmol/L） up to postoperative day 28.RESULTS：Blood glucose was lowest and serum insulin was highest in the islet＋UCMSCs group relative to the islet only group（both P 0.05）.The rate of normoglycaemia was better in the co-transplantation group than in the islet only group（P 0.05）.However,there were nodifferences in the parameters detected between the sham operation group and UCMSCs alone group,and no normoglycemic rats were found in the UCMSCs alone group.CONCLUSION：Co-transplantation of umbilical cord mesenchymal stem cells with islets is associated with enhanced islet graft activity and function.