高效液相色谱法测定卡西酮

Detecting cathinone with HPLC

目的建立卡西酮的高效液相色谱检测方法。方法采用UPLC-DAD分析方法。分析柱:Agilent ZorbaxSB-Phenyl柱(250mm×4.6mm,5μm),流动相为三氟乙酸(pH 3.5)∶乙腈为85∶15,流速0.2mL/min,检测波长254nm。结果卡西酮在0.5～1 000μg/mL浓度范围内线性关系良好R2=0.999 4,日内与日间保留时间和峰面积的标准偏差(RSD)均<1.06%,检出限为0.068μg/mL,平均回收率95.9%。结论本方法峰形好,分离度好,线性范围良好,回收率高,适用于刑事案件中卡西酮的定性定量分析。

Objective To establish a method for detecting cathinone with HPLC. Methods Using UPLC- DAD detection. The analytical column was Agilent Zorbax@ SB-Phenyl column （250mm x 4.6mm, 5 μm ） , and the mobile phase is trifluoracetic acid （pH 3.5 ） ：acetonitrile = 85：15, at a flow rate of 0. 2mL/min, with 254nm as the detection wavelength. Results This method has good linearity with the real value at the range from 0. 5 to 1 0001xg/mL, R2 = 0. 999 4. Both intra-day and inter-day precisions expressed by relative standard deviations of retention time and peak area were less than 1.06%. The detectable limit was 0. 068 μg/ mL. Average recovery was 95.9%. Conclusion This method has good peak shape, less mobile phase, good separation, good linearity and high recovery. This method was suitable for qualitative and quantitative analysis.