乌司他丁对脓毒症大鼠脾组织基因表达的影响

The effect of Ulinastatin on gene expression of septic rat＇s spleen tissue

目的应用基因芯片技术观察乌司他丁（Ulinastatin UTU预处理脓毒症大鼠脾组织基因表达谱的变化。方法将45只雄性Wistar大鼠按随机数字表法平均分为假手术组、脓毒症组和UTI组。采用盲肠结扎穿孔术复制脓毒症大鼠模型。UTI组于制模前1h肌肉注射UTI10万单位／kg；脓毒症组及假手术组肌注平衡液5ml／kg。采用RatRef-12大鼠表达谱基因芯片进行检测，用计算机软件筛选并分析比较脓毒症组、UTI组与假手术组大鼠脾组织基因表达的变化，并分析脓毒症组和UTI组表达基因的差异。结果在22523个基因中，与假手术组比较，脓毒症组大鼠脾组织差异表达基因共205个，占基因芯片总点数的0．910％；其中表达上调者98个，已知功能基因48个，仅在脓毒症组出现32个；表达下调者107个，已知功能基因64个，仅在脓毒症组出现34个。与假手术组比较，UTI组大鼠脾组织差异表达基因共197个，占基因芯片总点数的0．875％。其中表达上调者114个，已知功能基因35个，仅在UTI组出现19个；表达下调者83个，已知功能基因49个，仅在UTI组出现19个。结论UTI预处理可在一定程度上纠正部分脓毒症大鼠过度炎症反应及免疫抑制所致脾组织基因表达异常，在基因水平上对脾组织有保护作用。

Objective To explore the effect of UTI （Ulinastatin） preconditioning on gene expression profrles of spleen tissue in septic rats by DNA microarray technology. Methods Forty-five male Wistar rats were equally divided into sham group, sepsis group and UTI group by means of random number table. In UTI group the rats were treated with intramuscular injection of UTI（ l0s U/kg） one hour before cecal ligation and puncture. In sepsis group and sham group intramuscular balanced solution （5 ml/kg） was given. Cecal ligation and puncture was used to reproduce septic rat model. Gene expression profile was studied by using RatRef-12 Rat gene expression profile microarray to detect the changes in gene expression pattern of rat spleen tissue after cecal ligation and puncture. Then using related computer software was used to screen and analyze the relationship between the Sepsis/UTI group and sham group. Results In 22 523 genes,205 differential genes were found between sepsis group and sham group, accounting for 0. 910%. Among them 98 genes were up-regulated,with 48 known functional genes and 32 genes only showed in this group;107 genes were down-regulated, with 64 known functional genes and 34 genes only showed in it. 197 differential genes were found in UTI group and sham group, accounting for 0. 875%. Among them 114 genes were up-regulated ,with 35 known functional genes and 19 genes only showed in this group; 83 genes were down-regulated, with 49 known functional genes and 19 genes only showed in it. Conclusions Abnormal expression of genes in the spleen tissue of rats with sepsis owing to excessive inflammation and immune suppression were partly relieved by UTI preconditioning. UTI pretects spleen at genetic level.