摘要
目的:分析胃癌组织中miR-155的表达差异及其临床意义。方法:收集手术切除的52例胃癌标本及其癌旁组织,采用实时荧光定量-聚合酶链反应(RT-PCR)检测癌组织及癌旁组织标本miR-155的表达水平,并分析miR-155的表达与临床病理的关系。结果:RT-PCR结果显示,miR-155在胃癌组织中表达值(0.84±2.27)明显高于其配对癌旁组织中的表达值(0.28±0.56)(P<0.05);miR-155的表达水平与淋巴结转移有关,有淋巴结转移组明显高于无转移组(P<0.05);miR-155的表达与性别、年龄、肿瘤大小、侵犯层次、TNM分期及脉管侵犯无关,各参数的分组间差异无统计学意义(均P>0.05)。结论:miR-155在胃癌组织中呈高表达,且可能与胃癌的淋巴结转移有关。
Objective: To investigate the miR-155 expression in gastric cancer tissues and its clinical relevance. Methods: The surgical specimens of 52 cases of stomach cancer and their adjacent normal tissues were collected. The miR-155 expression level in gastric cancer and adjacent normal tissues were determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) technique, and the relations of miR-155 expression with the clinicopathologic factors of gastric cancer were analyzed. Results: The RT-PCR results showed that the miR-155 expression value in gastric cancer tissues (0.84+2.27) was significantly higher than that in their paired ad)acent normal tissues (0.28±0.56) (P〈0.05). The miR-155 expression level was associated with the lymph node metastases in gastric cancer patients, the miR-155 expression in patients with the lymph node metastases was higher than that in those without lymph node metastases (P〈0.05). The miR-155 expression level was irrelevant to the sex, age, tumor size, depth of invasion, and all the differences between the groups on these factors had no statistical significance (all P〉0.05).Conclusion: miR-155 has high expression level in gastric cancer tissues, and it may be relevant to gastric cancer lymph node metastasis.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2012年第10期1236-1239,共4页
China Journal of General Surgery
基金
江苏省卫生厅面上科研项目(H201220)
江苏省普通高校研究生科研创新计划项目(CXLX11_0742)
徐州市科技发展基金资助项目(XF10C080)