鼻咽癌血清NMR代谢组学研究

Serum NMR metabonomics of the nasopharyngeal carcinoma

目的:应用核磁共振氢谱和PLS-DA分析方法研究鼻咽癌患者血清的代谢组学变化。方法:临床正常人血清20例和鼻咽癌患者血清20例,采用日本电子600MHz超导核磁共振波谱仪进行检测,并运用PLS-DA分析方法,得出鼻咽癌患者血清代谢组的特征,探讨相关生化过程的改变。结果:鼻咽癌患者和正常人血清的代谢组学具有明显差异。鼻咽癌患者血清中的甲硫氨酸、牛磺酸和胆碱类物质的含量升高,而脂类、异亮氨酸、不饱和脂类、氧化三甲胺和糖类降低。结论:通过上述代谢物的变化可以区分正常人和鼻咽癌患者。这种基于核磁共振氢谱和PLS-DA分析的代谢组学方法可以为鼻咽癌的诊断提供可靠的代谢依据。

Objective：By using 1-H NMR and PLS-DA analysis method to research NPC serum levels＇s metabonomics changes.Methods：Select 20 cases clinical normal serum and 20 NPC 20 serum,with the 600 MHz Japanese electronics superconducting magnetic resonance spectroscopy testing instrument and PLS-DA.Results：NPC and normal serum＇s metabonomics levels had obvious difference.NPC serum＇s methionine,taurine and choline kind material were rised,and lipid,Isoleucine,trimethylamine oxide,sugar and unsaturated lipid reduced.Conclusion：Through the above metabolites changes can distinguish the difference between normal and NPC patients.This kinds of based on 1-H NMR and PLS-DA metabonomics analysis method for the diagnosis of NPC can provide reliable from molecular level.