普通小麦×硬粒小麦杂种蛋白质含量及其电泳分析

Protein Content and Its Gel Electrophoresis Analysis in Hybrids Derived from Crosses between Triticum aestivum and T.durum

本文分析了普通小麦×硬粒小麦杂种14个选系的籽粒蛋白质含量及7个选系籽粒氨基酸组成。科春_(14)×D_(213)杂种7个选系的平均蛋白质含量为18.45%,比双亲平均数(15.04%)高。肯尼亚×阿波罗各选系蛋白质含量比其亲本平均提高3.44%。籽粒中氨基酸含量随着籽粒蛋白质含量的提高而增加。选系 KA_(20)的蛋白质含量为20.13%,比母本肯尼亚(16.01%)高;其籽粒赖氨酸含量为0.4174,也高于肯尼亚(0.3172)。结果证明:把硬粒小麦的有益基因引入普通小麦,可以得到高蛋白质类型。亲本和杂种各系间电泳图谱也有某些差异,可以根据电泳图谱鉴别杂种。

The grain protein contents of 14 lines selected from the hybrids Triticum aestivum×T.durum and the amino acid components in seven of these lines were analysed.Themean value of grain protein content of the seven selected lines derived from Kechun_(14)×D_(213) was 18.45%,and higher than that of their parents(15.04%).And the averagevalue of grain protein content of another seven selected lines derived from Kenya×Applowas 18.42%,also higher than that of their parents.The grain amino acid content increased with the increase of grain protein content.For example,the protein content of a selected line KA_(20)(20.13%)was higher than that of its maternal Kenya(16.01%),andthe grain lysine content of KA_(20)(0.4174)was also higher than that of Kenya(0.3172).This experimented results indicates that high protein content type can be obtained bytransfering effective gene(s)of T.durum to common wheat.The above-mentioned lines also showed some differences in electrophoresis patterns.Itseems that we may identify hybrids according to their electrophoresis patterns.