17－β雌二醇对大鼠肝切除肝缺血再灌注损伤后残肝再生的影响

Effects of 17-15 estradiol on regeneration of the remnant liver after semi-hepatectomy with 45 mi- nutes of hepatic ischemia in rats

目的观察17－β雌二醇对大鼠肝切除肝缺血再灌注损伤后残肝再生的影响。方法雄性SD大鼠随机分为单纯50％肝切除组（R组）、50％肝切除肝缺血再灌注损伤组（R＋IR组）及17－β雌二醇处理组（E2＋R＋IR组）。观察术后7d生存率、血清谷丙转氨酶（ALT）、肝脏组织学及肝脏再生情况。结果与R＋IR组比较，17-B雌二醇显著延长大鼠生存率（80％比20％，P〈0．05），各时间点ALT水平显著下降（P〈0．05），病理损伤明显改善，再生肝重量（RLW）明显升高，在再灌注后2d残肝组织细胞核增殖抗原（Ki-67）、增殖细胞核抗原（PCNA）和细胞周期蛋白D1（Cyclin D1）表达显著增多。结论7-β雌二醇对大鼠肝切除肝缺血再灌注损伤后残肝的再生具有促进作用，其作用机制可能与诱导CyclinD1的高表达有关。

Objective To investigate whether 17q3estradiol can promote regeneration of the remnant liver after semi-hepatectomy with 45 minutes of hepatic ischemia in rats. Methods Male Sprague- Dawley rats were randomly allocated into 3 groups： R group, rats subjected to 50% hepatectomy alone; R ＋ IR group, rats subjected to 50% hepatectomy and 45min of hepatic ischemia; E2 ＋ R ＋ IR group, rats administration with 17-βestradiol. The 7d survival rate, serum alanine aminotransferase （ALT） level, liver histology and remnant liver regeneration were then investigated. Results Compared with R ＋ IR group, 17-βestradiol significantly improved the 7 d survival rate （80% vs 20%, P 〈 0. 05）, decreased ALT levels （P 〈 0. 05） and increased the RLW （ P 〈 0. 05 ）. The pathological changes at 12h after reperfusion were milder and the expression of Ki-67, proliferating cell nuclear antigen （PCNA） and Cyclin D1 in the 2d af- ter reperfusion were significantly higher in E2 ＋ R ＋ IR group Comparison to those in the R ＋ IR group. Conclusion 17-βestradiol could ＂promote regeneration of the remnant liver after semi-hepatectomy with 45 minutes of hepatic ischemia in rats. The high expression of Cyclin D1 may be one of the reasons.