叉状头／翅膀状螺旋转录因子Foxp3在BXSB狼疮小鼠小肠组织的表达

Expression of immune molecule forkhead box P3 in the small intestine of lupus-prone BXSB mice

目的观察免疫分子叉状头／翅膀状螺旋转录因子Foxp3和若干细胞因子在BXSB狼疮小鼠血清及小肠组织的水平变化，探讨与狼疮性肠炎发病有关的免疫机制。方法选取8周龄及16周龄雄性BXSB小鼠，并以8周龄C57BL／6雄性小鼠为正常对照，用实时定量聚合酶链反应（Real-time PCR）方法，检测Voxp3在小鼠小肠中的mRNA表达水平；用酶联免疫吸附试验（ELISA）检测小肠组织和血清白细胞介素（IL）-10、转化生长因子-β（TGF-β）、IL-4和干扰素．1（IFN-1）水平变化。结果与正常对照组比较，8周龄和16周龄BXSB狼疮小鼠回肠组织Foxp3 mRNA表达均明显降低（7．33±1．00、5．22±0．88比9．72±0．69，P〈0．001），而IL．10及TGF一8水平显著升高[IL一10：（60．94±4．93）、（71．00±9．66）ng／（g·protein）比（48．83±5．71）ng／（g·protein），P〈0．01；TGF—β：（37．77±4．21）、（39．53±4．47）ng／（g·protein）比（27．85±2．79）ng／（g·protein），P〈0．01]；而血清IL．4水平及IL-4与IFN-1比值则8周龄和16周龄BXSB小鼠均显著高于正常对照组[IL4：（39．18±5．47）、（48．30±6．16）ng／L比（27．60±3．50）ng／L，P〈0．01；IL-4／IFN-γ：0．61±0．09、0．68±0．12比0．45±0．08，P〈0．05]。结论小肠组织Foxp3mRNA表达降低和细胞因子网络紊乱，可能参与BXSB狼疮小鼠小肠炎症性病理改变的发病机制。

Objective To observed the expression of immune molecule forkhead/winged helix transcription factor forkhead box P3 （Foxp3） and certain cytokines in the small intestine and serum of BXSB mice in order to explore the potential role of Foxp3 in the pathogenesis of lupus associated enteritis in BXSB mice. Methods The expression of Foxp3 mRNA was detected by using quantitative real-time poly- merase chain reaction （Real-time PCR） in normal controls, 8-week-old and 16-week-aid BXSB male mice. Serum and tissue levels of interleukin （IL）-10, transformation growth factor beta （TGF-13）, IL-4 and interferon gamma （IFN-γ） were measured by enzyme-linked immunosorbent assay （ELISA）. Results As compared with normal controls, 8-week-old and 16-week-old BXSB mice all had significantly lower expression of ileum Foxp3 mRNA in the ileum （7.33 ± 1.00, 5.22 ±0. 88 vs. 9. 72 ±0. 69,P 〈0. 01 ）, and significantly higher levels of IL-10 and TGF-β in the ileum [IL-α （60. 94±4. 93）, （71.00 ±9. 66） ng/（g.protein） vs. （48. 83 ±5.71） ng/（g.protein）,P〈0.01; TGF-β（37.77 ±4.21）, （39.53 ±4.47） ng/（g.pro- tein） vs. （ 27.85 ± 2. 79 ） ng/（ g. protein）, P 〈 0. 01 ]. In addition, serum IL-4 level and the ratio of serum IL-4 to IFN-γwere all significantly higher in 8-week-old and 16-week-old BXSB mice than those in normal controls [IL-4： （39. 18 ±5.47）, （48.30±6. 16） ng/L vs： （27.60 ±3.50） ng/L,P 〈0. 01 ; IL-γ IFN-γ： 0.61±0.09, 0.68 ±0. 12, vs. 0.45 ±0.08,P〈0.05]. Conclusion Decreased Foxp3 mRNA expression and imbalanced eytokines could play a role in the pathogenesis of lupus associated enteritis in BXSB mice.